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Phospho-Rb (Ser780) polyclonal antibody

CAT: 0384-BZ16380-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BZ16380-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression. Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation. Specificity of different CDK/cyclin complexes has been observed in vitro and cyclin D1 is required for Ser780 phosphorylation in vivo.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
RB1; Retinoblastoma-associated protein; p105-Rb; pRb; Rb; pp110
Swiss Prot
P06400
Reactivity
Human, Mouse, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser780. AA range:751-800
Conjugation
Unconjugated
Applications
WB, IHC-F, IHC-P, ICC/IF, ELISA
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 106 kDa; Observed MW: 106 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-Rb in various lysates using Phospho-Rb antibody.
Host or Source
Rabbit

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