PHD1 polyclonal antibody

• Background :

  Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

• Structure Composition :

  Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Product Name Alternative :

  Estrogen-induced tag 6; HPH-3; PHD1

• Swiss Prot :

  Q96KS0

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  A synthesized peptide derived from human PHD1/prolyl hydroxylase

• Conjugation :

  Unconjugated

• Applications :

  WB, IHC-P, ICC/IF, FC

• Dilution :

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100

• Purity :

  Affinity Purified

• Modification :

  Unmodified

• Molecular Weight :

  Calculated MW: 44 kDa; Observed MW: 44 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  IgG

• Applications Notes :

  Western blot analysis of PHD1 in HeLa lysates; A549 lysates using PHD1 antibody.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4