PHD1 Polyclonal Antibody

• Background:

  PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.

• Synonyms:

  Egl nine homolog 2; prolyl hydroxylase; EGLN 2; EGLN2; EIT 6; EIT6; H P4H 1; H PH1; HPH1; HPH 3; HPH3; P4H1; PHD 1; PHD1; EGL nine C.elegans homolog 2; EGLN2_HUMAN.

• Gene ID:

  112398

• Cellular Locus:

  Cytoplasm, Nucleus

• Host:

  Rabbit

• Cross Reactivity:

  Mouse

• Immunogen:

  321-407/407

• Target:

  PHD1

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  112398

• Predicted Cross Reactivity:

  Human, Rat, Cow, Sheep