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RhoA/B/C polyclonal antibody

CAT: 0384-BZ16539-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BZ16539-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
RhoA, RhoB, RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Product Name Alternative
ARH12; ARH6; ARH9; ARHA; ARHA2; H12; RHO12; Transforming protein RhoA; RHOA; RHOB; RHOC
Swiss Prot
P61586/P62745/P08134
Reactivity
Human, Mouse, Rat
Immunogen
A synthesized peptide derived from human Rho A + B + C
Conjugation
Unconjugated
Applications
WB, IHC-P, ICC/IF, FC
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100
Purity
Affinity Purified
Modification
Unmodified
Molecular Weight
Calculated MW: 22 kDa; Observed MW: 22 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma using Rho A + B + C antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Host or Source
Rabbit

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