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Phospho-p53 (Ser15) polyclonal antibody

CAT: 0384-BZ16379-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BZ16379-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Product Name Alternative
TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Swiss Prot
P04637
Reactivity
Human, Rat
Immunogen
The antiserum was produced against synthesized peptide derived from human p53 around the phosphorylation site of Ser15. AA range:1-50
Conjugation
Unconjugated
Applications
WB, IHC-F, IHC-P, IP, ICC/IF, ELISA
Dilution
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 ELISA: 1/10000
Purity
Affinity Purified
Modification
Phosphorylated
Molecular Weight
Calculated MW: 44 kDa; Observed MW: 48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IgG
Applications Notes
Western blot analysis of Phospho-p53 in HeLa treated with HU lysates using Phospho-p53 antibody.The lane on the right is blocked with the Phospho- peptide.
Host or Source
Rabbit

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