HIF1 alpha polyclonal antibody

• Background:

  Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT) . This gene encodes the alpha subunit of HIF-1.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma

• Swiss Prot:

  Q16665

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377

• Conjugation:

  Unconjugated

• Applications:

  ICC/IF, WB, IHC-F, IHC-P, ELISA

• Dilution:

  WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

• Purity:

  Affinity Purified

• Modification:

  Unmodified

• Molecular Weight:

  Calculated MW: 93 kDa; Observed MW: 92-130 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of HIF1 alpha in LOVO lysates using HIF1 alpha antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4