Phospho-JAK2/3 (Tyr966/939) polyclonal antibody

• Background:

  Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.

• Structure Composition:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

• Product Name Alternative:

  Tyrosine-protein kinase JAK2/JAK3 (EC 2.7.10.2; Janus kinase 2/Janus kinase 3; JAK-2/JAK-3)

• Swiss Prot:

  O60674/P52333

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized Phospho peptide derived from human JAK2/3.at AA range: T966

• Conjugation:

  Unconjugated

• Applications:

  WB, ELISA

• Dilution:

  WB: 1/500-1/1000 ELISA: 1/10000

• Purity:

  Affinity Purified

• Modification:

  Phosphorylated

• Molecular Weight:

  Calculated MW: 131 kDa; Observed MW: 120-130 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG

• Applications Notes:

  Western blot analysis of Phospho-JAK2/3 in mouse liver, hela , mouse brain lysates using Phospho-JAK2/3 antibody.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4