JAK2 polyclonal antibody

• Background:

  JAK2 (Janus kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK2. JAK2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  JAK-2; JAK2; Janus Activating Kinase 2; Janus kinase 2 (a protein tyrosine kinase) ; Janus kinase 2; ; JTK10; kinase Jak2; OTTHUMP00000043260; THCYT3; Tyrosine protein kinase JAK2; Tyrosine-protein kinase JAK2

• Swiss Prot:

  O60674

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein.

• Conjugation:

  Unconjugated

• Applications:

  WB IF

• Dilution:

  WB:1:2000~1:5000 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodification

• Molecular Weight:

  ~ 130 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  JAK2 polyclonal antibody detects endogenous levels of JAK2 protein.

• Applications Notes:

  Western blot (WB) analysis of JAK2 pAb at 1:2000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:H9C2 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4