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IBA1 polyclonal antibody

CAT: 0384-BS90681-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS90681-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC) .
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
AIF1; AIF-1; IBA1; IRT-1; IRT1; Iba1
Swiss Prot
P55008
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human AIF1/IBA1 (NP_001614.3) .
Conjugation
Unconjugated
Applications
WB IHC-P IF/ICC
Dilution
WB 1:1:500-1:1000 ICC/IF 1:50-1:200 IHC-P 1:50-1:100
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 17 kDa
Storage Conditions
Store at +4°Cafter thawing. Aliquot store at -20°Cor -80°CAvoid repeated freeze / thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
IBA1 polyclonal antibody detects endogenous levels of IBA1 protein.
Applications Notes
Western blot analysis of extracts of THP-1 cells, using IBA1 antibody at 1:1000 dilution.
Host or Source
Rabbit

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