CD146 polyclonal antibody

• Background:

  Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146

• Swiss Prot:

  P43121

• Reactivity:

  Human

• Immunogen:

  Recombinant protein of human CD146.

• Conjugation:

  Unconjugated

• Applications:

  WB IP IF

• Dilution:

  WB: 1:2000~1:5000 IF 1:50~1:200 IP 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~110 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CD146 polyclonal antibody detects endogenous levels of CD146 protein.

• Applications Notes:

  Western blot (WB) analysis of CD146 polyclonal antibody at 1:2000 dilution Lane1:L02 cell membrane lysate (20ug) Lane2:A549 cell membrane lysate (20ug) Lane3:MCF-7 cell membrane lysate (20ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4