Factor B Polyclonal Antibody

• Background:

  The complement component proteins, C3, C4 and C5, are potent anaphylatoxins that are released during complement activation. Binding of these proteins to their respective G protein-coupled receptors, C3aR, C1R and C5aR, induces proinflammatory events, such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. Complement Factor B, also designated Properdin Factor B or PBF2, is part of the alternate pathway of the complement system and is cleaved by Factor D into two fragments: Ba and Bb. Bb combines with complement Factor 3b to produce the C3 or C5 convertase and plays a role in the differentiation and proliferation of preactivated B lymphocytes, lysis of erythrocytes, stimulation of lymphocyte blastogenesis and rapid spreading of peripheral blood monocytes. Ba is important in inhibiting the proliferation of preactivated B lymphocytes. Adipsin, also designated complement Factor D, is a serine protease that cleaves complement Factor B and may be involved in obesity. Factor H controls the function of the alternative complement pathway. FHR-1 (complement Factor H related protein 1) may play a role in lipid metabolism

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  AHUS4; B factor properdin; Factor B ; BF; BFD; C3 proaccelerator; C3 proactivator; C3/C5 convertase; CFAB; CFAB_HUMAN; CFB; Complement Factor B; Complement factor B Bb fragment; FB; FBI12; GBG; Glycine rich beta glycoprotein; Glycine-rich beta glycoprotein; H2 Bf; H2BF; PBF2; Properdin factor B

• Swiss Prot:

  P00751

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein containing a sequence corresponding to amino acids 26-259 of Complement factor B (CFB) (NP_001701.2) .

• Conjugation:

  Unconjugated

• Applications:

  WB IHC-P IF/ICC

• Dilution:

  WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 100 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  Factor B Polyclonal Antibody detects endogenous levels of Factor B protein.

• Applications Notes:

  Western blot analysis of extracts of various cell lines, using Complement factor B (CFB) antibody at 1:1000 dilution.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4