IκB-α (Phospho-S32/S36) polyclonal antibody

• Background:

  The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI

• Swiss Prot:

  P25963

• Reactivity:

  Human

• Immunogen:

  Synthetic phosphopeptide derived from human IκB-α around the phosphorylation site of Serine 32/Serine 36.

• Conjugation:

  Unconjugated

• Related Pathways:

  NF-kB Signaling, STING Signaling

• Applications:

  WB

• Dilution:

  WB 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Molecular Weight:

  ~ 39 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IκB-α (Phospho-S32/S36) polyclonal antibody detects endogenous levels of IκB-α protein only when phosphorylated at Ser32/Ser36.

• Applications Notes:

  Western blot (WB) analysis of IκB-α (Phospho-S32/S36) polyclonal antibody at 1:200 dilution Lane1:THP-1 whole cell lysate (30ug) Lane2:THP-1 treated with PMA (200nM, 30min) whole cell lysate (30ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4