CPT1A polyclonal antibody

• Background:

  The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  CPT1A; CPT1; CPT1-L; L-CPT1

• Swiss Prot:

  P50416

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length protein of human CPT1A.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB,1:500 - 1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 88 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CPT1A polyclonal antibody detects endogenous levels of CPT1A protein.

• Applications Notes:

  Western blot analysis of CPT1A expression in HL60 (A), THP1 (B), BT474 (C) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4