CDC25C polyclonal antibody

• Background:

  This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Product Name Alternative:

  CDC25C; CDC25; PPP1R60

• Swiss Prot:

  P30307

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC25C.

• Conjugation:

  Unconjugated

• Applications:

  WB, IHC, IF/ICC

• Dilution:

  WB,1:500 - 1:1000|IHC,1:100 - 1:200|IF/ICC,1:100 - 1:500

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 75 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  CDC25C polyclonal antibody detects endogenous levels of CDC25C protein.

• Applications Notes:

  Western blot analysis of CDC25C expression in rat brain (A), mouse lung (B), CT26 (C), PC12 (D), U87MG (E), Hela (F) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4