CD86 polyclonal antibody

• Background :

  This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.

• Structure Composition :

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative :

  B7-2; B7.2; B70; CD28LG2; LAB72; CD86

• Swiss Prot :

  P42081

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Recombinant fusion protein of human CD86 (NP_787058.4) .

• Conjugation :

  Unconjugated

• Applications :

  WB, IF/ICC

• Dilution :

  WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

• Purity :

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification :

  Unmodified

• Molecular Weight :

  ~ 72 kDa

• Storage Conditions :

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes :

  For research use only, not for use in diagnostic procedure.

• Specificity :

  CD86 polyclonal antibody detects endogenous levels of CD86 protein.

• Applications Notes :

  Western blot analysis of CD86 expression in mouse thymus (A), mouse spleen (B), rat spleen (C), rat lung (D) whole cell lysates.

• Host or Source :

  Rabbit

• CAS Number :

  9007-83-4