NRF-1 polyclonal antibody

• Background:

  Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells. The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis. In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities. While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology. NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis. NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy.

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

• Swiss Prot:

  Q16656

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant full length Human NRF-1.

• Conjugation:

  Unconjugated

• Applications:

  WB, IF/ICC

• Dilution:

  WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 70 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  NRF-1 polyclonal antibody detects endogenous levels of NRF-1 protein.

• Applications Notes:

  Western blot analysis of NRF1 expression in MCF7 (A), mouse testis (B) whole cell lysates.

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4