EIF3K polyclonal antibody

• Background:

  EIF3K (eukaryotic translation initiation factor 3 subunit K, muscle-specific gene M9 protein) is a widely expressed translation initiation factor that belongs to the eIF3 subunit K family. Translation initiation factor 3 (eIF3) is a multisubunit complex containing at least 12 subunits. eIF3 binds to the 40S ribosomal subunit, promotes the binding of methionyl-tRNAi and mRNA, and interacts with several other initiation factors to form the 40S initiation complex. eIF3K is the smallest subunit of eIF3 and it interacts with several other subunits of eIF3 and the 40S ribosomal subunit. eIF3K is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. eIF3K is distributed both in nucleus and cytoplasm and colocalizes with cyclin D3, a regulatory subunit of cyclin-dependent kinase 4 (Cdk4) .

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  Eukaryotic translation initiation factor 3 subunit K; eIF3k; Eukaryotic translation initiation factor 3 subunit 12; Muscle-specific gene M9 protein; PLAC-24; eIF-3 p25; eIF-3 p28; EIF3K; EIF3S12; ARG134; HSPC029; MSTP001; PTD001

• Swiss Prot:

  Q9UBQ5

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 50-100 of Human EIF3K.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB: 1:500~1:1000

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 25, 28KDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF3K polyclonal antibody detects endogenous levels of EIF3K protein.

• Applications Notes:

  Western blot (WB) analysis of EIF3K polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:The Testis tissue lysate of Mouse (40ug) Lane4:HEK293T whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4