EIF3H polyclonal antibody

• Background:

  Component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

• Product Name Alternative:

  EIF3H; EIF3S3; eIF3-gamma; eIF3-p40

• Swiss Prot:

  O15372

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human EIF3H (NP_003747.1) .

• Conjugation:

  Unconjugated

• Applications:

  WB IHC IP IF

• Dilution:

  WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200|IF,1:50 - 1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  40kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  EIF3H polyclonal antibody detects endogenous levels of EIF3H protein.

• Applications Notes:

  Western blot (WB) analysis of EIF3H pAb at 1:5000 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:The Pancreas tissue lysate of Mouse (40ug) Lane5:The Thymus tissue lysate of Rat (40ug) Lane6:PC12 whole cell lysate (40ug) Lane7:RAW264.7 whole cell lysate (40ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4