EIF3H polyclonal antibody

CAT: 0384-BS8655-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS8655-01Size:50 µL
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Background
Component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
EIF3H; EIF3S3; eIF3-gamma; eIF3-p40
Swiss Prot
O15372
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein of human EIF3H (NP_003747.1) .
Conjugation
Unconjugated
Applications
WB IHC IP IF
Dilution
WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200|IF,1:50 - 1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
40kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
EIF3H polyclonal antibody detects endogenous levels of EIF3H protein.
Applications Notes
Western blot (WB) analysis of EIF3H pAb at 1:5000 dilution Lane1:EC9706 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:The Pancreas tissue lysate of Mouse (40ug) Lane5:The Thymus tissue lysate of Rat (40ug) Lane6:PC12 whole cell lysate (40ug) Lane7:RAW264.7 whole cell lysate (40ug)
Host or Source
Rabbit

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