RNF111 polyclonal antibody

CAT: 0384-BS61642-01Size: 50 µLDry Ice: NoHazardous: No
CAT#:0384-BS61642-01Size:50 µL
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Background
Communication between cells during early embryogenesis establishes the basic organization of the vertebrate body plan. The early mammalian embryo is patterned by signals emanating from extraembryonic and embryonic signaling centers, most notably the anterior visceral endoderm (AVE) and the node, respectively. Nodal-related members of the transforming growth factor (TGF) β family regulate the induction of mesoderm, endoderm and mesendoderm. The different tissues form in response to the same signaling molecules, which may elicit differential responses through concentration-dependent effects, extracellular cofactors and antagonists, or factor potentiation. The nuclear protein Arkadia specifically potentiates the mesendoderm-inducing activity of a subset of TGFβ family members and interacts with Nodal, which mediates the function of Arkadia in node induction. Arkadia functions within extraembryonic tissues and is required to induce the node as an essential modulator of the nodal signalling cascade.The human gene for Arkadia maps to chromosome 15q21 and encodes a 441 amino acid protein. The murine gene for Arkadia maps to chromosome 9 39.0 cM and encodes a 989 amino acid protein.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
E3 ubiquitin-protein ligase Arkadia; RING finger protein 111; hRNF111; RING-type E3 ubiquitin transferase Arkadia; RNF111
Swiss Prot
Q6ZNA4
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding Human RNF111.
Conjugation
Unconjugated
Applications
WB
Dilution
WB: 1:500~1:1000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 120 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
RNF111 polyclonal antibody detects endogenous levels of RNF111 protein.
Applications Notes
Western blot (WB) analysis of RNF111 polyclonal antibody at 1:1000 dilution Lane1:Hela whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug)
Host or Source
Rabbit

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