JNK1/2/3 (phospho-T183) polyclonal antibody

• Background:

  The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced) .

• Structure Composition:

  Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  JNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9

• Swiss Prot:

  P45983/P45984/P53779

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic phosphopeptide derived from human JNK1 around the phosphorylation site of Threonine 183.

• Conjugation:

  Unconjugated

• Related Pathways:

  Immune Cell Signaling, SAPK JNK Signaling Cascades, Inhibition of Apoptosis, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, MAPK Erk in Growth and Differentiation Pathway, Mitochondrial Control of Apoptosis, ErbB HER Signaling, ErbB HER Signaling

• Applications:

  WB IHC IF

• Dilution:

  WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

• Purity:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Phosphorylated

• Molecular Weight:

  ~ 54 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  P-JNK1/2/3 (T183) polyclonal antibody detects endogenous levels of JNK1/2/3 protein only when phosphorylated at Thr183.

• Applications Notes:

  Western blot (WB) analysis of p-JNK1/2/3 (T183) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:K562 whole cell lysate (20ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Kidney tissue lysate of Mouse (20ug)

• Host or Source:

  Rabbit

• CAS Number:

  9007-83-4