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HSP60 (T547) polyclonal antibody

CAT: 0384-BS1179-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0384-BS1179-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
HSPs (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins, and the HSP 110 family. The constitutively expressed mitochondrial protein HSP 60 shares the ability to recognize and stabilize proteins during folding, assembly and disassembly with other HSP family members. The mitochondrial and cytosolic localization of HSP 60, combined with its binding and catalysis of folding of newly synthesized proteins destined for the mitochondrial matrix, classify this protein as a molecular chaperone. An additional role of HSP 60 is to act as a cell surface marker forr γ/δ T cell recognition.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; Chaperonin 60; CPN60; Heat shock protein 60; HSP-60; Hsp60; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein; HSPD1; HSP60
Swiss Prot
P10809
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide, corresponding to amino acids 511-560 of Human HSP60.
Conjugation
Unconjugated
Related Pathways
Mitochondrial Control of Apoptosis
Applications
WB IHC IF IP
Dilution
WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodified
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
HSP60 (T547) polyclonal antibody detects endogenous levels of HSP60 protein.
Applications Notes
Western blot (WB) analysis of HSP60 (T547) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:L02 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:C6 whole cell lysate (40ug)
Host or Source
Rabbit

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