NKRF mouse monoclonal antibody

• Background:

  NF-κB Repressing Factor (NKRF) is a constitutively expressed transcription factor that binds to the negative regulatory element within the promotor region of many genes that are transactivated by NF-κB (1) . Indeed, research studies have found NKRF to repress transcription of IL-8/CXCL8, iNOS, CXCL10, and IFN-γ (2-4) . In addition to regulating the expression of pro-inflammatory factors, NKRF has been shown to play a role in cell survival in the context of proteotoxic stress, through the regulation of rRNA processing and ribosome biogenesis (5) .

• Structure Composition:

  1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Product Name Alternative:

  NF-kappa-B-repressing factor; NFkB-repressing factor; Protein ITBA4; NKRF; ITBA4, NRF; 9606 NCBIHomo sapiens (Human)

• Swiss Prot:

  O15226

• Reactivity:

  Human, Mouse

• Immunogen:

  Human recombinant protein fragment corresponding to amino acids 1-296 of human NKRF (NP_060014) produced in E.coli.

• Conjugation:

  Unconjugated

• Applications:

  WB

• Dilution:

  WB 1:2000

• Purity:

  The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Modification:

  Unmodified

• Molecular Weight:

  ~ 90 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  IgG2a

• Applications Notes:

  Western blot (WB) analysis of NKRF mouse monoclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (30ug)

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4