SAMHD1 Mouse monoclonal antibody

• Background:

  SAM domain and HD domain-containing protein 1 (SAMHD1) is a negative regulator of the cell-intrinsic innate immune response. Research studies have identified mutations in SAMHD1 as a cause of Aicardi-Goutieres syndrome, an autoimmune disease characterized by elevated production of interferon-α and symptoms resembling congenital viral infection. SAMHD1 was identified as the restriction factor that renders most myeloid cells refractory to human immunodeficiency virus (HIV) infection. Expression of the viral protein Vpx in refractory cells targets SAMHD1 for ubiquitin-mediated degradation and relieves HIV restriction. SAMHD1 prevents autoimmunity and HIV infection by hydrolyzing intracellular deoxynucleoside triphosphates (dNTPs), thereby limiting inappropriate immune activation by self nucleic acid and inhibiting reverse transcription of the HIV genome.

• Structure Composition:

  PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

• Product Name Alternative:

  CHBL2; DCIP; HDDC1; MOP-5; SBBI88

• Swiss Prot:

  Q9Y3Z3

• Reactivity:

  Human, Monkey

• Immunogen:

  Full length human recombinant protein of human SAMHD1 (NP_056289) produced in HEK293T cell.

• Conjugation:

  Unconjugated

• Applications:

  FC, IF, IHC, WB

• Dilution:

  WB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100

• Purity:

  Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

• Modification:

  Unmodified

• Molecular Weight:

  ~ 72 kDa

• Storage Conditions:

  Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

• Notes:

  For research use only, not for use in diagnostic procedure.

• Specificity:

  SAMHD1 Mouse monoclonal antibody detects endogenous levels of SAMHD1 protein.

• Applications Notes:

  Western blot (WB) analysis of SAMHD1 Mouse monoclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate (30ug) Lane2:COS-7 whole cell lysate (30ug) Lane3:MCF-7 whole cell lysate (30ug)

• Host or Source:

  Mouse

• CAS Number:

  9007-83-4