RNA Aptamer Broccoli (sodium)

• UNSPSC Description:

  RNA Aptamer Broccoli (sodium) is a 49-nt-long aptamer that is substantially shorter than Spinach and Spinach2 and exhibits bright green fluorescence upon binding DFHBI or DFHBI-1T (soluble analogs of the fluorophore of green fluorescent protein). RNA Aptamer Broccoli (sodium) can be used to visualize RNA expression or localization in live cells. In vitro Broccoli exhibits a similar high folding efficiency as Spinach2, but exhibits markedly lower dependence on magnesium for folding and increased thermostability. Additionally, unlike Spinach2, Broccoli does not require the use of a tRNA scaffold to promote its folding in vivo.

• Target Antigen:

  Others

• Type:

  Oligonucleotides

• Related Pathways:

  Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/rna-aptamer-broccoli.html

• Solubility:

  10 mM in H2O

• Smiles:

  [RNA Aptamer Broccoli (sodium)]

• References & Citations:

  [1]Filonov GS, Moon JD, Svensen N, Jaffrey SR. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014;136(46):16299-16308. doi:10.1021/ja508478x

• Shipping Conditions:

  Room temperature

• Clinical Information:

  No Development Reported