4-Nitrophenyl phosphate (disodium hexahydrate)

• UNSPSC Description:

  4-Nitrophenyl phosphate (p-nitrophenyl phosphate) disodium hexahydrate is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. 4-Nitrophenyl phosphate disodium hexahydrate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].

• Target Antigen:

  Biochemical Assay Reagents

• Type:

  Biochemical Assay Reagents

• Related Pathways:

  Others

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/4-nitrophenyl-phosphate-disodium-hexahydrate.html

• Purity:

  99.80

• Solubility:

  DMSO : 10 mg/mL (ultrasonic;warming;heat to 80°C)|H2O : 100 mg/mL (ultrasonic)

• Smiles:

  O=[N+]([O-])C1=CC=C(C=C1)OP(O[Na])(O[Na])=O.[6H2O]

• Molecular Weight:

  371.14

• References & Citations:

  [1]Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015;71(Pt 2):199-205. |[2]Qi S, et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020;145(11):3871-3877. Int J Mol Sci. 2022 Feb 26;23(5):2604.|J Chromatogr A. 2023 Nov 17, 464511.|Am J Physiol Cell Physiol. 2019 Dec 1;317(6):C1115-C1127.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  -20°C (Powder, sealed storage, away from moisture)

• Clinical Information:

  No Development Reported

• CAS Number:

  333338-18-4