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4-Nitrophenyl phosphate

CAT: 0804-HY-116022-01Size: 5 mgDry Ice: NoHazardous: No
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CAT#:0804-HY-116022-01Size:5 mg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.4-Nitrophenyl phosphate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].
CAS Number
330-13-2
Product Name Alternative
P-Nitrophenyl phosphate
UNSPSC
12352200
Hazard Statement
H302, H315, H319, H335
Target
Biochemical Assay Reagents
Type
Reference compound
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/4-nitrophenyl-phosphate.html
Purity
99.48
Solubility
DMSO : 100 mg/mL (ultrasonic) |H2O : 100 mg/mL (ultrasonic)
Smiles
O=P(O)(O)OC(C=C1)=CC=C1[N+]([O-])=O
Molecular Formula
C6H6NO6P
Molecular Weight
219.09
Precautions
H302, H315, H319, H335
References & Citations
[1]Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015 Feb;71 (Pt 2) :199-205. |[2]Qi S , et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020 Jun 7;145 (11) :3871-3877.
Shipping Conditions
Room Temperature
Storage Conditions
-20°C, 3 years; 4°C, 2 years (Powder)
Scientific Category
Biochemical Assay Reagents
Clinical Information
No Development Reported

Chemical Information

4-Nitrophenyl Phosphate

4-nitrophenyl phosphate is an aryl phosphate resulting from the mono-esterification of phosphoric acid with 4-nitrophenol. It has a role as a mouse metabolite. It is functionally related to a 4-nitrophenol. It is a conjugate acid of a 4-nitrophenyl phosphate(2-).

CAS Number330-13-2
PubChem CID378
IUPAC Name(4-nitrophenyl) dihydrogen phosphate
Molecular FormulaC6H6NO6P
Molecular Weight219.09
XLogP0.3
Topological Polar Surface Area113
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count6
Rotatable Bond Count2
Heavy Atom Count14
Formal Charge0
Complexity250
SMILES
C1=CC(=CC=C1[N+](=O)[O-])OP(=O)(O)O
InChI
InChI=1S/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12)
InChIKey
XZKIHKMTEMTJQX-UHFFFAOYSA-N
Chemical Structure
2D Structure
2D structure of 4-Nitrophenyl Phosphate
CAS: 330-13-2
CID: 378
Formula: C6H6NO6P
MW: 219.09
Interactive 3D Structure
Loading 3D structure...
View on PubChemRaw 3D data
Computed Properties
PropertyValue
Molecular Weight219.09
XLogP30.3
Hydrogen Bond Donor Count2
Hydrogen Bond Acceptor Count6
Rotatable Bond Count2
Exact Mass218.99327391
Monoisotopic Mass218.99327391
Topological Polar Surface Area113 Ų
Heavy Atom Count14
Formal Charge0
Complexity250
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
Covalently-Bonded Unit Count1
Compound Is CanonicalizedYes

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