4-Nitrophenyl phosphate

4-Nitrophenyl phosphate

• CAS Number: 330-13-2
• UNSPSC Description: 4-Nitrophenyl phosphate (p-Nitrophenyl phosphate) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases.4-Nitrophenyl phosphate is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm[1].
• Target Antigen: Biochemical Assay Reagents
• Type: Reference compound
• Related Pathways: Others
• Field of Research: Others
• Assay Protocol: https://www.medchemexpress.com/4-nitrophenyl-phosphate.html
• Solubility: 10 mM in DMSO
• Smiles: O=P(O)(O)OC(C=C1)=CC=C1[N+]([O-])=O
• Molecular Weight: 219.09
• References & Citations: [1]Lountos GT, et al. Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate. Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):199-205. |[2]Qi S , et al. Development of a facile and sensitive method for detecting alkaline phosphatase activity in serum with fluorescent gold nanoclusters based on the inner filter effect. Analyst. 2020 Jun 7;145(11):3871-3877.
• Shipping Conditions: Room temperature
• Clinical Information: No Development Reported