Infliximab (Remicade) Pharmacokinetic ELISA

Infliximab (Remicade) Pharmacokinetic ELISA

• Description: Infliximab (Remicade®) is a chimeric monoclonal antibody against tumour necrosis factor alpha (TNF- α). Infliximab neutralizes the biological activity of TNF- α by binding with high affinity to the soluble (free floating in the blood) and transmembrane (located on the outer membranes of T cells and similar immune cells) forms of TNF-α, and inhibits or prevents the effective binding of TNF-α with its receptors.
• CAS Number: 7732-18-5
• Gene ID: 7124
• Reaction Volume: 15 µL
• Target Antigen: Infliximab (Remicade)
• Detection Method: Direct sandwich ELISA
• Assay Principle: Quantification of Infliximab in biological matrices
• Assay Protocol: This assay employs the sandwich enzyme immunoassay technique. Anti- Infliximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Infliximab present in biological matrices is bound by the immobilized anti- Infliximab antibody. After washing away any unbound substances, enzyme linked anti- Infliximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Remicade® (domain residing in Fc portion of the Remicade® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Infliximab present in test samples. The color development is stopped and the intensity of the color is measured.
• Assay Performance Time: 2.5 Hours
• Sample Type: Serum Plasma
• Detection Range: 25ng/mL - 0.78ng/mL
• Precision: <10%, <10%
• Sensitivity: Infliximab
• Limit Of Detection: 0.78 ng/mL
• Components: Coated microtiter plate, 96 wells Calibrator diluent. - 1.8mL Calibrator 12ul 10X wash buffer - 25mL Assay buffer - 50mL 1000X detection reagent - 17ul TMB - 12mL TMB stop solution - 12mL Plate sealers - 3
• Shipping Conditions: Blue Ice
• Storage Conditions: -20°C
• Shelf Life: 12 months