Etanercept (Enbrel) Pharmacokinetic ELISA

Etanercept (Enbrel) Pharmacokinetic ELISA

• Description: Etanercept (trade name Enbrel®) is a protein drug used to treat autoimmune diseases by adsorbing tumor necrosis factor (TNF; a soluble inflammatory cytokine). Etanercept is a fusion protein produced through expression of recombinant DNA by linking the extracellular ligand-binding portion of TNFRSF1B to the Fc component of human immunoglobulin G1 (IgG1). It reduces the effect of naturally present TNF, functioning as a decoy receptor that binds to TNF. Etanercept is indicated for the treatment of moderate to severe rheumatoid arthritis (RA), psoriatic arthritis, ankylosing spondylitis, and moderately to severely active polyarticular juvenile idiopathic arthritis. Serum concentration of Enbrel® may predict some clinical outcome during maintenance therapy.
• CAS Number: 7732-18-5
• Gene ID: 7124
• Reaction Volume: 15 µL
• Target Antigen: Etanercept (Enbrel)
• Detection Method: Direct sandwich ELISA
• Assay Principle: Quantification of Etanercept in biological matrices
• Assay Protocol: This assay employs the sandwich enzyme immunoassay technique. Capture antibody is coated onto a 96 well microplate. Calibrator and test samples are pipetted into the appropriate wells. Etanercept present in biological matrices is bound by the immobilized anti-Etanercept antibody. After washing away any unbound substances, enzyme linked anti-Etanercept antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Etanercept present in test samples. The color development is stopped and the intensity of the color is measured.
• Assay Performance Time: 2.5 Hours
• Sample Type: Serum Plasma
• Detection Range: 50ng/mL - 1.56ng/mL
• Precision: <10%, <10%
• Sensitivity: Etanercept
• Limit Of Detection: 1.5 ng/mL
• Components: Coated microtiter plate, 96 wells Calibrator diluent. - 1.8mL Calibrator 12ul 10X wash buffer - 25mL Assay buffer - 50mL 1000X detection reagent - 17ul TMB - 12mL TMB stop solution - 12mL Plate sealers - 3
• Shipping Conditions: Blue Ice
• Storage Conditions: -20°C
• Shelf Life: 12 months