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Villin Antibody Cocktail

CAT: 0013-GTR18082861-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082861-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
UniProt
P09327
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 179-311) was used as the immunogen for this Villin antibody cocktail.
Clonality
Monoclonal
Clone
VIL1/1314 + VIL1/2376
Conjugation
Unconjugated
Field of Research
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Villin antibody cocktail should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa + Mouse IgG1, kappa

UniProtKB · P09327

Villin-1

VILI_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09327
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VIL1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R9A7, Q53S11, Q96AC8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

Actin cappingActin-binding

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Ligand

Calcium

Cellular component

Cell projectionCytoplasmCytoskeleton

PTM

Phosphoprotein

Domain

Repeat

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