CD99 Antibody

CAT: 0013-GTR18082329-02Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18082329-02Size:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

CD99 is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, it may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of the protein. [Wiki]

UniProt

P14209

Reactivity

Human

Immunogen

Recombinant full-length human protein was used as the immunogen for the CD99 antibody.

Clonality

Monoclonal

Clone

CDLA99

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the CD99 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. Not suitable for rat samples testing.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

UniProtKB · P14209

CD99 antigen

CD99_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P14209
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CD99
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A6NIW1, O00518, Q6ICV7

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

PTM

GlycoproteinPhosphoprotein

Cellular component

Membrane

Domain

SignalTransmembraneTransmembrane helix

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