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AKT1 Antibody

CAT: 0013-GTR18082083-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082083-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB-beta or RacPK-beta) and Akt 3 (also designated PKB-gamma or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a Pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-beta tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
UniProt
P31749
Reactivity
Human
Immunogen
A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.
Clonality
Monoclonal
Clone
AKT1/2491
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Titering of the AKT1 antibody may be required for optimal performance.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2b, kappa

UniProtKB · P31749

RAC-alpha serine/threonine-protein kinase

AKT1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P31749
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AKT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
B2RAM5, B7Z5R1, Q9BWB6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCarbohydrate metabolismGlucose metabolismGlycogen biosynthesisGlycogen metabolismNeurogenesisSugar transportTranslation regulationTransport

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembraneMitochondrionNucleus

Molecular function

Developmental proteinKinaseSerine/threonine-protein kinaseTransferase

Disease

Disease variantProto-oncogene

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