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Thrombomodulin Antibody / CD141

CAT: 0013-GTR18081779-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081779-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma) . Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.
UniProt
P07204
Reactivity
Human
Immunogen
Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.
Clonality
Monoclonal
Clone
THBD/1591
Conjugation
Unconjugated
Field of Research
Cardiovascular Research; Immunology & Inflammation
Purification
Protein G affinity chromatography
Dilution
ELISA: 2-4ug/ml (order BSA/azide-free format), Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.
Tested Applications
ELISA, IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2b, kappa

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