S100A9 Antibody

CAT: 0013-GTR18081519-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18081519-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This mAb stains the cytoplasm of macrophages and histiocytes in hematopoietic organs, Kupffer's cells of the liver and Langerhan's cells of the skin. It also stains the mantle zone B-lymphocytes of the lymph node and spleen, spermatogonia, and chief cells of the stomach. S100A9 is expressed by macrophages in acutely inflamed tissues and in chronic inflammation. It is detected in peripheral blood leukocytes, in neutrophils and granulocytes. It is present at sites of vascular inflammation. S100A9 is also expressed in epithelial cells constitutively or induced during dermatoses. S100A9 is a Calcium-binding protein. It has antimicrobial activity towards bacteria and fungi. It is important for resistance to invasion by pathogenic bacteria. It up-regulates transcription of genes that are under the control of NF-kappa-B. S100A9 plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) . It promotes tubulin polymerization when unphosphorylated. It also promotes phagocyte migration and infiltration of granulocytes at sites of wounding. It plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1.

UniProt

P06702

Reactivity

Human, Rat

Immunogen

Human peripheral blood monocyte components were used as the immunogen for the S100A9 antibody.

Clonality

Monoclonal

Clone

47-8D3

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the S100A9 antibody should be determined by the researcher.1. For staining of formalin-fixed, paraffin-embedded tissues, digest with Trypsin at 1mg/ml PBS, 15 min at RT

Tested Applications

FACS, IF, IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG1, kappa

UniProtKB · P06702

Protein S100-A9

S10A9_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P06702
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: S100A9
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: D3DV36, Q6FGA1, Q9NYM0, Q9UCJ1

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Molecular function

AntimicrobialAntioxidant

Biological process

ApoptosisAutophagyChemotaxisImmunityInflammatory responseInnate immunity

Ligand

CalciumMetal-bindingZinc

Cellular component

Cell membraneCytoplasmCytoskeletonMembraneSecreted

PTM

MethylationPhosphoproteinS-nitrosylation

Domain

Repeat

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