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S100A9/MRP14 Antibody

CAT: 0013-GTR18225291Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18225291Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
S100A9/MRP14 Antibody
Product Name Alternative
S100A9, 60B8AG, Calgranulin B, CFAG, CAGB, Calgranulin-B, Calprotectin L1H subunit, CGLB, L1Ag, MRP14, NIF, p14, Protein S100-A9, LIAG, MAC387, MRP-14
Reactivity
Human
Immunogen
Peptide with sequence C-DTNADKQLSFEEF, from the internal region of the protein sequence according to NP_002956.1.
Target
S100A9 / MRP14
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
0.5 mg/ml
Dilution
IHC, IHC-P (2.5 μg/ml), Peptide-ELISA (1:8000), WB (0.3 - 1 μg/ml)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1) . The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16;280 (50) :41521-9.; PMID: 16216873) . Recommended concentration: 0.3-1 ug/ml.
Tested Applications
ELISA, IHC, IHC-P, WB
Host or Source
Goat
Preservative
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin

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