TRAcP Antibody / TRAP / ACP5

CAT: 0013-GTR18081485-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18081485-01Size:20 µg

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Description

It recognizes a protein of 35kDa, which is identified as tartrate-resistant acid phosphatase (TRAcP) . It exists as two isoforms (5a and 5b) . This mAb reacts with both the isoforms. Serum TRAcP 5a is secreted by macrophages and dendritic cells and increased in many patients of rheumatoid arthritis. Serum TRAcP 5b is produced from osteoclasts and elevated during bone resorption. TRAcP is an iron containing glycoprotein, which catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. It is the most basic of the acid phosphatases and is the only form not inhibited by L (+) -tartrate. TRAcP is synthesized as a latent proenzyme and is activated by proteolytic cleavage and reduction. Normally, TRAcP is highly expressed by osteoclasts, activated macrophages, neurons and endometrium during pregnancy. Expression of TRAcP is increased in certain pathological conditions such as Leukemic Reticuloendotheliosis (Hairy Cell Leukemia), Gaucher s Disease, HIV-induced Encephalopathy, Osteoclastoma and in osteoporosis and metabolic bone diseases. Anti-TRAcP antibody labels the cells of Hairy Cell Leukemia (HCL) with a high degree of sensitivity and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts.

UniProt

P13686

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full-length human protein was used as the immunogen for the TRAcP antibody.

Clonality

Monoclonal

Clone

SPM601

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the TRAcP antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2b, kappa

UniProtKB · P13686

Tartrate-resistant acid phosphatase type 5

PPA5_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P13686
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ACP5
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: TR-AP
EC number: 3.1.3.2
Processing: Precursor
Secondary accessions: A8K3V2, Q2TAB1, Q6IAS6, Q9UCJ5, Q9UCJ6, Q9UCJ7

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Disease variant

PTM

Disulfide bondGlycoprotein

Molecular function

Hydrolase

Ligand

IronMetal-binding

Cellular component

LysosomeSecreted

Domain

Signal

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Alternative Products

Catalog	Name
V2795-20UG	TRAcP Antibody / TRAP / ACP5
F47709-0.4ML	TRAP Antibody / TRAcP / ACP5
V3534IHC-7ML	Recombinant TRAcP Antibody / TRAP / ACP5
RQ5412	ACP5 Antibody / TRAcP
T8170-24	TRACP/PAP/ACP5 (Tartrate Resistant Acid Phosphatase, TRAP)
33-455	TRAcP Antibody [ACP5/1070]
A03277	Anti-TRAP ACP5 Antibody
NBP3-07651-20ug	Rabbit Monoclonal TRACP/PAP/ACP5 Antibody (ACP5/2336R)
NBP2-45293-0.02mg	Mouse Monoclonal TRACP/PAP/ACP5 Antibody (ACP5/1070)
NBP2-41178-0.025mg	Rabbit Polyclonal TRACP/PAP/ACP5 Antibody