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ICAM-3 Antibody

CAT: 0013-GTR18081334-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081334-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (WS: IV & V) . Its epitope localizes in the D2 extracellular domain and is resistant to neuraminidase and proteases. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signalling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb inhibits primary mixed lymphocyte culture (MLC) but not secondary MLC, cytotoxicity or proliferation induced by mitogens. It blocks binding of NK1-L16 stimulated T cells to L cells expressing CD50. This mAb is excellent for staining of formalin/paraffin tissues.
UniProt
P32942
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for the ICAM-3 antibody.
Clonality
Monoclonal
Clone
101-1D2
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the ICAM-3 antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Tested Applications
FACS, IF, IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG2a, kappa

UniProtKB · P32942

Intercellular adhesion molecule 3

ICAM3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P32942
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ICAM3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
ICAM-3
EC number
—
Processing
Precursor
Secondary accessions
Q6PD68
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell adhesionPhagocytosis

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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