ICAM-3 Antibody

CAT: 0013-GTR18081333-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0013-GTR18081333-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This mAb is excellent for staining of formalin/paraffin tissues.

UniProt

P32942

Reactivity

Human

Immunogen

Stimulated human leukocytes were used as the immunogen for the anti-ICAM-3 antibody.

Clonality

Monoclonal

Clone

SPM505

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein G affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the anti-ICAM-3 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Mouse IgG2b, kappa

UniProtKB · P32942

Intercellular adhesion molecule 3

ICAM3_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P32942
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ICAM3
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: ICAM-3
EC number: —
Processing: Precursor
Secondary accessions: Q6PD68

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell adhesionPhagocytosis

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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Alternative Products

Catalog	Name
V2607-20UG	ICAM-3 Antibody
NBP2-50434	Mouse Monoclonal ICAM-3/CD50 Antibody (ICAM 3.2)
MBS9503815-01	CD50/ICAM-3 Antibody
V7853SAF-100UG	CD50 Antibody / ICAM-3
V2606-100UG	Anti-ICAM-3 Antibody
36841-05111	Human ICAM-3 Antibody
33-400	ICAM-3 Antibody [SPM505]
NBP2-50434B	Mouse Monoclonal ICAM-3/CD50 Antibody (ICAM 3.2) [Biotin]
NBP2-50434PCP	Mouse Monoclonal ICAM-3/CD50 Antibody (ICAM 3.2) [PerCP]
NBP2-50434PE	Mouse Monoclonal ICAM-3/CD50 Antibody (ICAM 3.2) [PE]