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PAX7 Antibody

CAT: 0013-GTR18081037-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18081037-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The PAX gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the PAX family of proteins have been linked to disease and cancer in humans. PAX7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that PAX7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding PAX7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.
Clonality
Monoclonal
Clone
PAX7/497
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Protein G affinity chromatography
Dilution
Immunofluorescence: 0.5-1ug/ml, Flow cytometry: 0.5-1ug/10^6 cells, Western blot: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Tested Applications
FACS, IF, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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