POU5F1 Antibody / OCT4

• Description:

  OCT4 (Octamer-binding protein 4), also called POU5F1, is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  Q01860

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment of the human protein was used as the immunogen for the POU5F1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  OCT4/8098R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the POU5F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This POU5F1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the POU5F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the POU5F1 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human seminoma tissue with POU5F1 antibody (clone OCT4/8098R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.