CD21 Antibody

• Description:

  Recognizes a protein of 140kDa, which is identified as the complement receptor 2 (CR2) or CD21. This protein is expressed strongly on mature B cells, follicular dendritic cells and weakly on immature thymocytes and T lymphocytes. In B-cell ontogeny, CD21 appears after the pre-B-stage, is maintained during peripheral B-cell development and is lost upon terminal differentiation into plasma cells. CD21 expression is also gradually lost after stimulation of B cells in vitro. CD21 functions as receptor for C3d, C3dg and iC3b Complement components, for EBV and for IFNalpha. CD21 binds to CD23 and associates with CD19, CD81 and Leu13 to form a large signal-transduction complex involved in B cell activation.

• Specifications:

  ELISA (Order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P20023

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  The human B cell line SB was used as the immunogen for the CD21 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  CR2/6683

• Applications:

  ELISA, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD21 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD21 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD21 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Surface

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD21 antibody (clone CR2/6683) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.