Napsin A Antibody

• Description:

  Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

• Specifications:

  Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  O96009

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 50-150) from the human protein was used as the immunogen for the Napsin A antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  NAPSA/7165R

• Type:

  Recombinant

• Applications:

  WB, IHC-P

• Purity:

  Protein A affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the Napsin A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Napsin A antibody is available for research use only.

• Storage Conditions:

  Aliquot the Napsin A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Napsin A antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasm

• Image Legend:

  Western blot testing of human kidney tissue lysate with Napsin A antibody. Expected molecular weight: 38-45 kDa.