CD107a Antibody / LAMP1

• Description:

  Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107a) and LAMP-2 (also designated CD107b) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans-Golgi network and are transported intracellularly via a pathway that is distinct from the Clathrincoated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of Thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P11279

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein sequence (within amino acids 200-400) from the human protein was used as the immunogen for the CD107a antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  LAMP1/7457

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD107a antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD107a antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD107a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD107a antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, Cell membrane

• Image Legend:

  IHC staining of FFPE human prostate tissue with CD107a antibody (clone LAMP1/7457) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.