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Recombinant CD27 Antibody

CAT: 0800-V4221SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V4221SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P26842
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human full-length protein was used as the immunogen for the recombinant CD27 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
LPFS2/8574R
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the recombinant CD27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant CD27 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant CD27 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant CD27 antibody should be determined by the researcher.
Location
Cell Surface
Image Legend
Western blot testing of human Raji and Ramos cell lysate with recombinant CD27 antibody. Predicted molecular weight ~29 kDa but may be observed at higher molecular weights due to glycosylation.

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