CD3e Antibody

• Description:

  Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P07766

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant partial protein (within amino acids 1-200) from the human protein was used as the immunogen for the CD3e antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  C3e/8116R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the CD3e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD3e antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD3e antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD3e antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface, Cytoplasm

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD3e antibody (clone C3e/8116R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.