CD3e Antibody

• Description :

  Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt :

  P07766

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A recombinant partial protein (within amino acids 1-200) from the human protein was used as the immunogen for the CD3e antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG κ

• Clone :

  C3e/8116R

• Type :

  Recombinant

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the CD3e antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CD3e antibody is available for research use only.

• Storage Conditions :

  Aliquot the CD3e antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the CD3e antibody should be determined by the researcher.

• CAS Number :

  9000-83-3

• Location :

  Cell surface, Cytoplasm

• Image Legend :

  IHC staining of FFPE human tonsil tissue with CD3e antibody (clone C3e/8116R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.