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Ubiquitin Antibody

CAT: 0013-GTR18082139-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18082139-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ubiquitin is a small (8.5 kDa) regulatory protein found in most tissues of eukaryotic organisms. The addition of ubiquitin to a substrate protein is called ubiquitination or less frequently ubiquitylation. Ubiquitination affects proteins in many ways: it can mark them for degradation via the proteasome, alter their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination involves three main steps: activation, conjugation, and ligation, performed by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s), respectively. The result of this sequential cascade is to bind ubiquitin to lysine residues on the protein substrate via an isopeptide bond, cysteine residues through a thioester bond, serine and threonine residues through an ester bond, or the amino group of the protein's N-terminus via a peptide bond. [Wiki]
UniProt
P0CG48, P62987, P0CG47, P62988, P62979
Reactivity
Human
Immunogen
A portion of amino acids 1-119 from the human protein was used as the immunogen for the Ubiquitin antibody.
Clonality
Monoclonal
Clone
PBQN-1
Conjugation
Unconjugated
Field of Research
Epigenetics, NF-kB, Neuroscience, Signaling Pathways
Purification
Protein G affinity chromatography
Dilution
Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
The stated application concentrations are suggested starting points. Titration of the Ubiquitin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Tested Applications
IHC-P, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
Mouse IgG1, kappa

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