Rabbit anti Human beta-2 glycoprotein 1

Rabbit anti Human beta-2 glycoprotein 1

• Background: In bidimensional immunoelectrophoresis a single precipitin line is obtained and shows a reaction of full identity with the purified immunogen. The antiserum does not react with any other protein component of Human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) to identify the presence of beta-2 glycoprotein 1 or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserumin non-precipitating antibody-binding techniques as ELISA and Western blotting if proper controls are included.
• CAS Number: 9007-83-4
• UniProt: P02749
• Host: Rabbit
• Clone: Polyclonal
• Source: Beta-2 glycoprotein 1 has a molecular weight of 40,000. The mean concentration in adult serum is about 200 μg/mL. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Assay Principle: Precipitation assays. In immunoelectrophoresis use 2 μl Human plasma or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells. In electroimmunodiffusion use 1 percent antiserum in the gel. Measured by quantitative precipitin analysis. The amount of beta2 glycoprotein 1 precipitated by 1 mL antiserum is approximately 0.2 mg.
• Form: Delipidated, heat inactivated, lyophilized, stable whole serum; No preservative added; Total protein and IgG concentrations in the antiserum are comparable to those of; Normal pooled rabbit serum; No foreign proteins added; Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Storage Conditions: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C; Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance of the antiserum Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day