Anti-Hu CD49c APC

Anti-Hu CD49c APC

• Background: CD49c / Integrin alpha 3 is a type I transmembrane glycoprotein proteolytically cleaved into two disulfide linked chains. It noncovalently associates with CD29 (integrin beta 1) to form the VLA-3 complex, an adhesion receptor for extracellular matrix components (fibronectin, laminin 1, laminin 5, entactin, and collagen). It is expressed on adherent cells, mainly on fibroblasts, epithelial cells and endothelial cells.
• Specifications: The mouse monoclonal antibody ASC-1 recognizes an extracellular epitope of CD49c (integrin alpha 3), a transmembrane glycoprotein composed of disulfide linked 125 kDa and 30 kDa chains, and expressed on adherent cell lines and to a lesser extent on T and B cells and monocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human SSC-9 cell line (squamous cell carcinoma)
• Target Antigen: CD49c
• Isotype: Igg1 κ
• Clone: ASC-1
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Skubitz AP, Bast RC, Wayner EA, Letourneau PC, Wilke MS: Expression of alpha 6 and beta 4 integrins in serous ovarian carcinoma correlates with expression of the basement membrane protein laminin. Am J Pathol. 1996 May148(5):1445-61., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=wilke+ms+letourneau+pc ,*Pattaramalai S, Skubitz KM, Skubitz AP: A novel recognition site on laminin for the alpha 3 beta 1 integrin. Exp Cell Res. 1996 Feb 1222(2):281-90., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=pattaramalai+s+skubitz+km,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.