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Hematoxylin, PROBE

CAT: 0246-AR-6521-03Size: 250 mLDry Ice: NoHazardous: No
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CAT#:0246-AR-6521-03Size:250 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This uniquely formulated Probe hematoxylin is an excellent counterstain for in situ DNA hybridization procedure.
Applications
IHC
Assay Principle
1. After chromogen staining, wash slide 2-3X with de/di water, 2X with 2. Apply Probe hematoxylin for 2-3 minutes; wash with tap water 5-7X. 3. Soak the slides in Immuno Automation buffer (ImmunoBioScience AR-6561), PBS or any buffer with pH 7.4-8 for 2-3 minutes. Hematoxylin changes color from purple red to blue. 4. Wash slide 2-3X with de/di water. 5. The slides can be mounted with aqueous mounting medium (ImmunoHistoMount (AR-6503) or equivalent) for chromogens like AEC, DAB, Fast red, BCIP/INT, or Super Fast red. DAB and Super Fast can be mounted with Organic mounting mediums like Organo mount / limonene (AR-6504) or equivalent after dehydrating the tissue with alcohols and organic solvents.
Shipping Conditions
Ambient Temperature
Storage Conditions
Room Temperature

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