AEC Peroxidase Chromogen Substrate Kit

• Description:

  Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC).

• Applications:

  IHC, ICC

• Assay Principle:

  (THIS KIT CONTAINS approximately 166 X CHROMOGEN, When diluted will make approx. 425 ml)) 1. To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well. 2. Add one drop of reagent C chromogen, mix well. 3. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country’s regulations. Application: IHC, ICC 1. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/ deionized water. 2. Add few drops of ready to use AEC reagent, incubate at RT or 37ºC for 5-10 minutes. 3. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water. 4. Add counterstain compatible with AEC, we use hematoxylin 30-60 seconds (cat. # AR-6521). 5. Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water. 6. Mount slide with aqueous mounting medium, we use our IHM (cat. # AR-6503) please follow the protocol for mounting medium.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C